Epilobium hirsutum Linn. belongs to family Onagraceae, commonly known as great willowherb. E. hirsutum widely used traditional medicine. Despite its popular medicinal utilization, there were no definite phyto-physicochemical data of E. hirsutum has been reported so far for its pharmacognostical standardization. Thus, the research study was designed to investigate the phyto-physicochemical aspects of E. hirsutum. The various physicochemical parameters like ash values, extractive values, volatile oil content, moisture content and fluorescence analysis of E. hirsutum were determined for ascertaining the quality of the crude drug. The preliminary qualitative, as well as quantitative phytochemical analysis and high performance thin layer chromatography (HPTLC) of E. hirsutum, were carried out. The various physicochemical parameters of E. hirsutum were ascertained. The phytochemical analysis reveals the presence of carbohydrates, sugar, sterols, triterpenoids, anthraquinone glycosides, saponin glycoside, flavonoids, tannins and phenolic compounds in various extracts of E. hirsutum. The methanolic and aqueous extracts of leaves of E. hirsutum contained a high amount of total phenols and flavonoids as compared to the stem. The HPTLC fingerprinting of various phytochemical in methanol and aqueous extract of E. hirsutum were reported. The present finding can provide useful information regarding establishing pharmacognostic standards for identification, assessing purity, standardization and preparation of monograph of E. hirsutum.
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